I suppose the quickest way to get some small measure on this question
of to what extent spammers remove addresses on 5xx codes is to
honeypot some addresses, wait a while until they're being spammed, and
then begin returning 5xx codes for them and see if it makes any
measurable difference in the traffic.
The hard part there is "honeypotting" in a meaningful way soas not to
overly bias the sample.
I'll guess it'd take a few weeks to run such an experiment full cycle.
--
-Barry Shein
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